Assay capabilities & Specifications

 

Below is a list of assay capabilites and specifications.
For more background, please visit our resources page.

Fully customisable panels

Our off-the-shelf panels include whole transcriptome and surrogate panels for humans.

We can create new, fully custom panels for you, either as subsets of already existing panels, or by designing new probes. This can be include any isoform, variant, or mutation (SNP or fusion gene) for RNA or DNA.

Only minute amounts of sample needed

TempO-Seq runs directly from tissue or cell lysates without prior RNA extraction, purification, or cDNA synthesis. This drastically increases sensitivity, as RNA extraction is typically a step during which a lot of the RNA loss occurs, and only minute amounts of sample are needed for a successful assay run. Importantly, the assay is based on hybridisation of specific probes, which is a highly efficient and conserved molecular process, further enhancing TempO-Seq assay sensitivity.

Example types of input:

  • 2-10mm^2 of a 4 micron FFpe section or needle aspirate biopsies

    • can typically be H&E or antibody stained, as long as reagents are nuclease-free

    • however, input can also be smaller (LCM or similar)

  • sorted fixed cells (ICS-FACS)

  • Laser-capture microdissected (LCM) samples from ffpe tissue

  • whole blood

TempO-Seq has provided good results from many “difficult” or “low input” samples that are difficult to profile with other established technologies. Find out more about different sample requirements here, and please feel free to speak to us if you are unsure about your sample type or would like to know more.

High sensitivity and accuracy

As the assay circumvents the steps of RNA extraction/purification (which can result in significant RNA loss), reverse transcription (which is carried out by an enzyme that is evolutionarily designed to introduce errors), and does not require pre-amplification (which can induce bias), TempO-Seq offers excellent accuracy.

Based on highly efficient and sensitive hybridisation of specific probes to regions of interest within a gene, the assay is capable of detecting a lower threshold of ~ 16 molecules and offers high reproducibility down to 10 cells (or ~ 10 pg of RNA input).

Read TempO-Seq publications here.

High throughput and affordable

Based on the assay biochemistry, TempO-Seq enables high sample multiplexing on the sequencer - enabling the screening of large patient cohorts with fully customisable and highly multiplexed markers quickly and inexpensively.

Please get in touch below if you would like to discuss a project or get a quote.