Frequently asked questions.

BioClavis supports both kit-based and service-based TempO-Seq workflows. For a quotation, email info@bioclavis.co.uk or use the Enquiries page.

A typical TempO-Seq service project is completed in approximately 4–6 weeks after BioClavis receives the samples. Timing can vary with project scope, sample type and assay requirements.

Pricing depends on project size, panel content, sample number and whether you are purchasing kits or using BioClavis services. TempO-Seq is designed to reduce the cost of transcriptome analysis, with larger studies generally benefiting most from multiplexing. Contact BioClavis for a quotation.

Yes. BioClavis supports research internationally and works through a global distributor network. Visit Our Distributors or contact BioClavis for the best route in your region.

Sample preparation and submission information is available through the BioClavis Customer Page. If you need access or are unsure which form applies to your project, please contact BioClavis.

FASTQ files generated for service projects are made available as part of project delivery. Use the Customer Page for customer resources, or contact BioClavis if you need access instructions for your project files.

No. TempO-Seq can work directly from crude lysates and does not require nucleic acid extraction, purification or cDNA synthesis for supported workflows.

TempO-Seq supports a broad range of inputs, including cell or organoid lysates, isolated RNA, whole blood, dried blood spots, FFPE tissue and other low-input or clinically relevant samples. If your sample type is not listed, contact BioClavis to discuss compatibility.

TempO-Seq can work across a wide range of RNA inputs. For purified RNA workflows, BioSpyder guidance identifies 100 ng/µL as an ideal target concentration, while other TempO-Seq workflows can use substantially lower input amounts depending on sample type and assay design.

Charged slides are generally preferred because tissue adheres more securely and there is less concern about sample loss. Uncharged slides can also be used when appropriate, including with a coverslip.

TempO-Seq supports highly multiplexed assays ranging from focused panels with tens of targets to panels with tens of thousands of genes, up to whole-transcriptome content. BioClavis offers whole-transcriptome, surrogate and custom panel options.

Yes. BioClavis can design fully customisable panels around transcript variants, mutations, isoforms or other targets of interest. Contact BioClavis to discuss a custom panel.

S1500+ is a surrogate transcriptome panel designed to cover known biological pathways using a focused set of genes. Whole-transcriptome panels are designed to target all protein-coding genes, providing broader expression coverage.

Panel manifests are available through customer resources. Visit the Customer Page and use the manifests area to find available panel files.

TempO-Seq data analysis is substantially simplified compared with conventional RNA-Seq. BioClavis can provide gene-count CSV files and can also support custom bioinformatics, including exploratory analysis, differential expression, pathway analysis and other downstream workflows.

Yes. TempO-Seq data can be used for pathway-level analysis and tools such as Connectivity Map (CMap) or BMDExpress 2, depending on the study design and analysis goals.

TempO-Seq data is typically aligned and delivered as raw counts without a single imposed normalisation method. Multiple normalisation approaches can be used depending on the experimental design. A published comparison of TempO-Seq normalisation methods is available through NCBI.

BioSpyder documentation describes quantifying raw counts from aligned reads with the QuasR R package using default parameters. Your exact project output and analysis route can depend on the assay and delivery workflow.

A count table is required for quality-control and analysis tools, while a sample sheet is optional. Uploading a sample sheet can preserve plate-order sample names and lets the Description column be used to designate replicate groups for PCA.

Use the Description column in the sample sheet to identify replicates. Give samples in the same replicate group the same label, such as Liver, Kidney or Group1, and upload the sample sheet with the count table.

Alignment time depends on the number and size of FASTQ files. BioSpyder guidance gives a typical estimate of about one minute per five million reads, although heavy system load can increase the time. Check Alignment History for current status.

Alignment can fail during FASTQ validation or while mapping reads. A FASTQ-validation failure usually indicates a formatting issue in one or more files, while an incomplete alignment indicates a mapping-stage failure. If the problem persists, use the BioClavis Enquiries page for support.

Upload speed depends on file size and your internet connection. Large FASTQ files can take time to transfer. If files still will not upload after an extended period, contact BioClavis for support.

TempO-Seq Portal treats paired samples as independent samples during analysis.

The recommended sequencing depth is approximately 2–3 million reads per sample for the miRNome Panel.