Clinical research services

BioClavis is a spinout of BioSpyder Technologies, leveraging the TempO-Seq transcriptomic/genomic platform technology in the context of drug & biomarker discovery and precision medicine.

Usability features

TempO-Seq offers critical usability features that render it particularly suitable for clinical research and the real-world implementation of precision medicine. Sample inputs can be minute amounts of FFPE tissue or whole blood enabling unprecedented access to molecular analysis of precious and limited samples. TempO-Seq can analyse the whole transcriptome (off-the-shelf panel) or fully customisable panels of genes, single nucleotide polymorphisms (SNPs), fusion genes, or other mutations, ranging from hundreds to thousands of markers per panel (unlimited gene multiplexing). Due to the underlying TempO-Seq biochemistry, sample multiplexing per sequencing run is high and therefore, our assay is cheaper than RNA-seq or even qPCR at scale. For more information, see our assay specifications below.

TempO-Seq enables efficient and affordable molecular profiling from small and precious clinical samples.

Simplified analysis

In addition to usability features and cost reduction, bioinformatics analysis is often a significant and prohibitive use of time and resources. TempO-Seq data analysis is greatly simplified and outputs are counts per gene spreadsheets. TempO-SeqR, and online tool developed specifically for user-friendly data exploration, enable further analysis. BioClavis also offers bespoke in-depth analysis as a service solutions.

Partnering

In addition to clinical tests currently in our development and commercialisation pipeline, we are interested in partnering for the discovery of new biomarkers and the clinical development of diagnostic tests. We offer genomic/transcriptomic services for academic researchers as customers or collaborators and are happy to work with you on the development and commercialisation of new diagnostic or biomarker panels.

Assay capabilities & Specifications

Our off-the-shelf panels include whole transcriptome and surrogate panels for humans.

We can create new, fully custom panels for you, either as subsets of already existing panels, or by designing new probes. This can be include any isoform, variant, or mutation (SNP or fusion gene) for RNA or DNA.

TempO-Seq runs directly from tissue or cell lysates without prior RNA extraction, purification, or cDNA synthesis. This drastically increases sensitivity, as RNA extraction is typically a step during which a lot of the RNA loss occurs, and only minute amounts of sample are needed for a successful assay run. Importantly, the assay is based on hybridisation of specific probes, which is a highly efficient and conserved molecular process, further enhancing TempO-Seq assay sensitivity.

Example types of input:

• 2-10mm^2 of a 4 micron FFpe section or needle aspirate biopsies

  • can typically be H&E or antibody stained, as long as reagents are nuclease-free

  • however, input can also be smaller (LCM or similar)

• sorted fixed cells (ICS-FACS)

• Laser-capture microdissected (LCM) samples from ffpe tissue

• whole blood

TempO-Seq has provided good results from many “difficult” or “low input” samples that are difficult to profile with other established technologies. Find out more about different sample requirements here, and please feel free to speak to us if you are unsure about your sample type or would like to know more.

As the assay circumvents the steps of RNA extraction/purification (which can result in significant RNA loss), reverse transcription (which is carried out by an enzyme that is evolutionarily designed to introduce errors), and does not require pre-amplification (which can induce bias), TempO-Seq offers excellent accuracy.

Based on highly efficient and sensitive hybridisation of specific probes to regions of interest within a gene, the assay is capable of detecting a lower threshold of ~ 16 molecules and offers high reproducibility down to 10 cells (or ~ 10 pg of RNA input).

Read TempO-Seq publications here.

Based on the assay biochemistry, TempO-Seq enables high sample multiplexing on the sequencer - enabling the screening of large patient cohorts with fully customisable and highly multiplexed markers quickly and inexpensively.

Please get in touch below if you would like to discuss a project or get a quote.

Get in touch

Please complete the form below to discuss clinical research projects with us.