Assay capabilities & Specifications

Below is a list of assay capabilites and specifications.
For more background, please visit our resources page.

Our off-the-shelf panels include:

• whole transcriptome

  • human, mouse, and rat

• Surrogate transcriptome

  • human, mouse, rat, and zebrafish

  • bioinformatically designed to include 70-80% of pathways covered by the whole transcriptome with only ~4000 probes (maximally diverse expression and minimal co-regulation)

  • particularly useful for large pathway-level screens of biological activity

We can create new, fully customised panels for you, either as subsets of already existing panels, or by designing new probes. This can be include any isoform, mutation (SNP or fusion gene) and species, as long as you are able to provide a reference transcriptome/genome or reliable FASTQ files.

TempO-Seq runs directly from tissue or cell lysates without RNA extraction, purification, or cDNA synthesis. This drastically increases sensitivity, as RNA extraction can cause loss of RNA or introduce size bias. The assay is based on hybridisation of specific probes to the sample’s RNA in situ, which is a highly efficient and conserved molecular process. Only minute amounts of sample are needed for a successful assay run.

Example types of input:

• Crude cell lysates in a 96- or 384-well format

• 2-10 mm^2 of a 4 micron FFpe section

  • can be H&E or antibody stained, as long as reagents are nuclease-free

  • can also be smaller (e.g. LCM), please speak to us.

• sorted fixed cells (ICS-FACS)

• Laser-capture microdissected (LCM) samples from ffpe tissue

• whole blood

• whole zebrafish embryo lysate

TempO-Seq has provided good results from many “difficult” or “low input” samples that are difficult to profile with other established technologies. Our sample requirements can be found here, but please feel free to speak to us if you are unsure about your sample type or would like to know more.

As the assay circumvents the steps of RNA extraction/purification (which can result in significant RNA loss), reverse transcription (which is carried out by an enzyme that is evolutionarily designed to introduce errors), and does not require pre-amplification (which can induce bias), TempO-Seq offers excellent accuracy.

Based on highly efficient and sensitive hybridisation of specific probes to regions of interest within a gene, the assay is capable of detecting a lower threshold of ~ 16 molecules and offers high reproducibility down to 10 pg of RNA input (single cell equivalent).

Read TempO-Seq publications here.

Based on the assay biochemistry, TempO-Seq enables high sample multiplexing on the sequencer - enabling the screening of large patient cohorts quickly and inexpensively.